Batman & the Lizard is a 1999 direct-to-video superhero animated feature film, the second based on Spider-Man: The Animated Series, serving as a stand-alone sequel to Spider-Man: Mask of the Ghost set on Earth-92131.
Since his last encounter against Spider-Man two years ago, the Lizard has found himself a home in the sewers and started a surrogate family with two ordinary lizards that he mutated into lizard creatures like himself, using the Neogenic Recombinator. He has his minions kidnap Curt Connor's wife Margaret and son Billy and mutates them into lizard creatures as well. However the Lizard realises that in their lizard forms, his wife and son are just ravages.
The Lizard decides to enlist the help of Connor's old colleague, Dr. Burton, to fix the problem. Dr. Burton admits that Connors had modified the Neogenic Recombinator and only Peter Parker knows how it works now.
The Lizard attacks a restaurant that Peter Parker and Mary Jane Watson are at and kidnaps Mary Jane. Peter changes into his Spider-Man costume and gives chases but fails to stop the Lizard who takes Mary Jane into his lair.
Spider-Man locates the Lizard's lair but the Lizard has already mutated Mary Jane into a lizard creature, intending to blackmail Peter into fixing the flaws of the Neogenic Recombinator. Spider-Man is forced to leave with the intention of returning as Peter to fix the Neogenic Recombinator.
The mutated Mary Jane is able to convince Billy to help her escape. The lizard creatures give pursuit and corner her, just before Peter arrives, saying he can fix the Neogenic Recombinator. Instead Peter uses the machine to turn Mary Jane, Margaret and Billy back to normal. Angered at the double cross, the Lizard attacks Peter who escapes to change into his Spider-Man that he had left nearby. The Lizard takes the opportunity to turn Mary Jane back into a lizard creature it turns out Peter had also rigged the device to explode, starting a rapidly spreading fire as the Lizard traps Mary Jane. The Lizard orders Dr. Burton to fix the machine but he attempts to escape, only to be killed by falling rubble. The Lizard decides to just kill Mary Jane but Peter returns as Spider-Man and fights him. With Margaret and Billy about to be killed by the lizard creatures, Curt Connors takes control of the Lizard's body to stop them, telling Spider-Man to save his wife and son. Spider-Man and Mary Jane get Margaret and Billy to safety. Despite his efforts, Spider-Man is unable to save the Lizard from plummeting further into the sewers, to his apparent death.
The Lizard is revealed to have survived and escaped with his lizard creatures. With Curt Connors in control, he is teaching them to tame their animalistic nature.
- Christopher Daniel Barnes as Peter Parker/Spider-Man
- Joseph Campanella as Curt Connors/Lizard
- Sara Ballantine as Mary Jane Watson
- Liselle Loren as Margaret Connors
- Toby Scott Ganger as Billy Connors
- George Dzundza as Dr. Burton
- Julie Bennett as Aunt May