Spider-Man: Mystery of the Spider-Woman is a 2007 direct-to-video animated film based on the animated series Spider-Man: The Animated Series, serving as a stand-alone sequel to both Spider-Man: Mask of the Ghost and Spider-Man & the Lizard set on Earth-92131.
A new heroine has arrived in New York whose identity is a mystery—even to Spider-Man. During patrol, the Spider-Man spots her trying to stop one of the Doctor Octopus' shipments on New York's interstate, using a web shooter to send the Doc Ock's truck with its driver off the bridge. Spider-Man saves the driver from falling to his death.
Spider-Man must figure out who the Spider-Woman is and stop two familiar enemies, Doctor Octopus and the Kingpin, from selling illegal weapons to the fictional nation of Symkaria. The two employ Jerry Franklin, a gangster, to provide protection.
- Christopher Daniel Barnes as Peter Parker/Spider-Man
- TBA as Jessica Drew
- TBA as Julia Carpenter
- TBA as Mattie Franklin
- TBA as Spider-Woman
- Efrem Zimbalist Jr. as Doctor Octopus
- TBA as Jerry Franklin
- Hank Azaria as Venom
- Dawnn Lewis as Detective Terri Lee
- Roscoe Lee Brownie as Kingpin
- Julie Bennett as Aunt May